Methods

The EURL for FMD, among other missions, gathers data and information on the methods of diagnosis and differential diagnosis used in the different National Laboratories. A list of methods inspired by the WOAH official methods is available on this website.
WOAH Terrestrial Manual 2022

 

All methods used for FMD detection, including commercial kits, must be WOAH-based methods validated according to international standards.

You will find attached the most recent version of the WOAH terrestrial manual chapter regarding foot-and-mouth disease.

 

FDMV detection and typing - Decision tree

 

Diagnostic methods for FMD detection and typing are described in the WOAH Terrestrial Manual.

This document sums up the diagnostic strategy as a decision tree describing the workflow for implementation of the different methods prescribed. 

All methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.

Methodology
Samples Treatment

 

This SOP is the WOAH-based method currently used at the EURL for FMD.

All other methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.

Methodology
Virus Isolation

 

This SOP is the WOAH-based method currently used at the EURL for FMD detection.

All other methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.

Methodology
Duplex one-step real-time RT-PCR assays for diagnosis of foot-and-mouth disease

 

This SOP is the WOAH-based method currently used at the EURL for FMD detection.

All other methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.

Methodology
Antigen detection ELISA

 

This SOP is the WOAH-based method currently used at the EURL for FMD detection and typing.

All other methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.

Methodology
Standard operating procedure FMD VP1 amplification

 

This SOP is the WOAH-based method currently used at the EURL for FMD detection.

All other methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.

Methodology