Virus Isolation

 

The test relies on the in vitro detection of the infectivity of foot-and-mouth disease virus through the observation of a cytopathic effect (CPE) following the inoculation of susceptible cells.

If there is a clinical suspicion of foot-and-mouth disease in pigs, a differential diagnosis with swine vesicular disease is undertaken directly using two different cell types.

If a CPE is observed, the culture supernatant is collected and additional tests should be carried out to confirm FMDV. Find this document here.

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