EURL
European Union Reference Laboratory for
Foot-and-Mouth disease
LRUE Fièvre Aphteuse
URL:
https://eurl-fmd.anses.fr
Background type:
color
Intro [EN]:
The European Union Reference Laboratory organises Proficiency Tests (PTs) on detection and typing of Foot-and-Mouth disease virus in accordance with the Regulation (EU) No 2017/625.
PT panels for FMDV and SVDV detection are established for virus isolation, RT-PCR, Antigen-ELISA and VP1 sequencing. PT panels for FMD antibody detection are established for FMDV NSP antibodies, FMDV SP antibodies and SVDV antibodies detection by ELISAs or/and virus neutralisation test.
The proficiency test (PT) for FMD virus and SVD virus and antibody detection is organised annually. All EU national reference laboratories (NRLs) and other official laboratories of the network are invited to participate.
Intro [EN]:
The European Union Reference Laboratory yearly organizes a workshop, especially intended for the EU national reference laboratories (NRLs) and other official laboratories of the network.
The purpose of these workshops is to inform the participants about the activities of the EURL, to present and discuss the results of the PT, to present and discuss scientific research projects and to exchange information on EU legislation, methods and other relevant subjects.
Registration to the next workshopIntro [EN]:
The EURL for FMD, among other missions, gathers data and information on the methods of diagnosis and differential diagnosis used in the different National Laboratories.
A list of methods inspired by the WOAH official methods is available on this website.
Intro [EN]:
The EURL for FMD supports the functions of National Laboratories by storing and supplying reagents and materials for use in diagnosis of foot-and-mouth disease such as virus and/or inactivated antigens, standardised sera, cell lines and other reference reagents.
A list of reagents and material is available on this website.
Display Name:
Foot-and-Mouth disease
Title [FR]:
Méthodes
Title [EN]:
Methods
Title [FR]:
Réactifs disponibles et matériaux de référence
Title [EN]:
Available reagents and reference materials
Methods Background:
Materiau Background:
Code AT Internet:
603911
Nom page d'accueil (AT):
Accueil LRUE FA
Intro [EN]:
The European Union Reference Laboratory organizes each year trainings specifically intended for the EU national reference laboratories (NRLs) and other official laboratories of the network.
The purpose of these sessions is to implement the further training of experts in laboratory diagnosis with the goal to harmonize the diagnostic techniques at the European and International levels.
WRL - The Pirbright Institute
List of Foot-and-mouth disease reagents available at the WRL the Pirbright Institute can be found here.
Whole genome sequencing of FMDV using Nextera XT kit from Illumina
This kit is used for the sequencing of the whole genome of Foot-and-Mouth Disease Virus.
Sanger sequencing of FMDV
This procedure describes the sequencing of the VP1 gene of FMDV using a Sanger sequencing approach and the SeqStudio Genetic Analyzer from Life technologies.
(Triplex) One-Step real-time rtPCR for the detection of the viral RNA of Senecavirus A
This SOP describes the procedure for the detection of Senecavirus A (SVA) RNA using a (triplex) real-time RT-PCR.
rtRT-PCR for the detection of SAT2/V FMDV
This real-time RT-PCR is a molecular tool for detection of Foot-and-mouth disease virus topotype SAT2/V.
rtRT-PCR for the detection of O/EA-3 FMDV
This real-time RT-PCR is a molecular tool for detection of Foot-and-mouth disease virus topotype O/EA-3 (from West and North Africa).
2025 Proficiency test on Foot and Mouth Disease
2025 Proficiency test on Foot and Mouth Disease
Test reports:
► Online test report for panel 1,
► Online test report for panel 2,
► Online test report for panel 3,
Beware that the test reports are not linked with leila. Therefore be careful to keep the link you will get to be able to come back and modify your entries.
Nevertheless once validated you will not be able to modify your test report(s).
Keep in mind that you need to upload the test reports joint in a zip-file in leila website.
Outbreak of Foot-and-Mouth disease in Slovakia
An outbreak of Foot-and-Mouth Disease has been reported in Slovakia in domestic cattle for the first time in 50 years. Cases have been detected in three farms located in the southern part of the country. This is the third case in the EU in 2025 after the outbreaks in Germany in January and in Hungary in March. Restriction zones have been set up around the farms. The identification of the serotype is pending.
For more information: Slovakia records first foot-and-mouth cases, minister says | Reuters; WAHIS
Outbreak of Foot-and-Mouth disease in Hungary
An outbreak of Foot-and-Mouth Disease has been reported in Hungary in domestic cattle. The outbreak is located in the North-west of the country in the administrative zone of Győr-Moson-Sopron. This is the second case in the EU in 2025 after the outbreak in Germany in January. Restriction zones have been set up around the outbreak and animals have been slaughtered. Following sequencing, the strain responsible for the infection was identified : O/ME-SA/PanAsia2/ANT10.
For more information: https://portal.nebih.gov.hu/-/megjelent-a-ragados-szaj-es-koromfajas-betegseg-magyarorszagon; WAHIS
rtRT-PCR for the detection of O/ME-SA/SA-2018 FMDV
This real-time RT-PCR is a molecular tool for detection of Foot-and-mouth disease virus lineage O/ME-SA/SA-2018, as it is an emerging lineage in South Asia since 2018.