EURL staphylococci
URL:
https://eurl-staphylococci.anses.fr
Background type:
color
Display Name:
Coagulase Positive Staphylococci
Methods Background:
Materiau Background:
CPS Characterisation
List of methods available at EURL CPS
Detection of Staphylococcal enterotoxins (SE): list, reference sequence of se genes, reference sequence of SE proteins -
Current knowledge on staphylococcal enterotoxins, their role in food poisoning, and advances in detection and classification, are summerised in the review by Franjus et al 2026
WGS pipeline:
1- StarFlow
2- Galaxy Workflow 1 v1 and NEW Galaxy Workflow 1 v2
3- NEW Galaxy Workflow 2 v1
Whole genome sequencing (WGS) protocols and methods:
- NEW: Recommendations for WGS wet lab and dry lab methods
- Protocol for manual DNA extraction and semi automated DNA extraction
- Protocol for Illumina library preparation on a Miseq
- Comparison of WGS with classical typing methods (Vingadassalon et al 2024)
- SNP threshold for the analysis of CPS outbreaks (Duchez et al 2025)
- Genomic analysis tool for the detection of enterotoxins coding genes (NAuRA - Merda et al 2019)
Conventional multiplex PCR (sea to see and ser and seg to sej and sep)
Multiplex RT-PCR: LSA-INS-1294