LRUE Fièvre Aphteuse
The European Union Reference Laboratory organises Proficiency Tests (PTs) on detection and typing of Foot-and-Mouth disease virus in accordance with the Regulation (EU) No 2017/625.
PT panels for FMDV and SVDV detection are established for virus isolation, RT-PCR, Antigen-ELISA and VP1 sequencing. PT panels for FMD antibody detection are established for FMDV NSP antibodies, FMDV SP antibodies and SVDV antibodies detection by ELISAs or/and virus neutralisation test.
The proficiency test (PT) for FMD virus and SVD virus and antibody detection is organised annually. All EU national reference laboratories (NRLs) and other official laboratories of the network are invited to participate.
The European Union Reference Laboratory yearly organizes a workshop, especially intended for the EU national reference laboratories (NRLs) and other official laboratories of the network.
The purpose of these workshops is to inform the participants about the activities of the EURL, to present and discuss the results of the PT, to present and discuss scientific research projects and to exchange information on EU legislation, methods and other relevant subjects.
Registration to the next workshopThe EURL for FMD, among other missions, gathers data and information on the methods of diagnosis and differential diagnosis used in the different National Laboratories.
A list of methods inspired by the WOAH official methods is available on this website.
The EURL for FMD supports the functions of National Laboratories by storing and supplying reagents and materials for use in diagnosis of foot-and-mouth disease such as virus and/or inactivated antigens, standardised sera, cell lines and other reference reagents.
A list of reagents and material is available on this website.
The European Union Reference Laboratory organizes each year trainings specifically intended for the EU national reference laboratories (NRLs) and other official laboratories of the network.
The purpose of these sessions is to implement the further training of experts in laboratory diagnosis with the goal to harmonize the diagnostic techniques at the European and International levels.
WOAH Terrestrial Manual 2022
All methods used for FMD detection, including commercial kits, must be WOAH-based methods validated according to international standards.
You will find attached the most recent version of the WOAH terrestrial manual chapter regarding foot-and-mouth disease.
FDMV detection and typing - Decision tree
Diagnostic methods for FMD detection and typing are described in the WOAH Terrestrial Manual.
This document sums up the diagnostic strategy as a decision tree describing the workflow for implementation of the different methods prescribed.
All methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.
Samples Treatment
This SOP is the WOAH-based method currently used at the EURL for FMD.
All other methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.
Virus Isolation
This SOP is the WOAH-based method currently used at the EURL for FMD detection.
All other methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.
Duplex one-step real-time RT-PCR assays for diagnosis of foot-and-mouth disease
This SOP is the WOAH-based method currently used at the EURL for FMD detection.
All other methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.
Antigen detection ELISA
This SOP is the WOAH-based method currently used at the EURL for FMD detection and typing.
All other methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.
Standard operating procedure FMD VP1 amplification
This SOP is the WOAH-based method currently used at the EURL for FMD detection.
All other methods used, including commercial kits, must be WOAH-based methods validated according to international standards and used successfully in the annual PT.
Suspicion of foot-and-mouth disease outbreak in Rodrigues Island
For more information : https://promedmail.org/promed-post/?id=8247240
Recent publications
Please, find the recent publications here : https://sitesv2.anses.fr/en/minisite/lrue-fievre-aphteuse/publications
Recent publications
Find recent publications in this website
Please, find the recent publications here : https://sitesv2.anses.fr/en/minisite/lrue-fievre-aphteuse/publications