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LISTERIA MONOCYTOGENES MOLECULAR SEROTYPING: AMPLIFICATION OF THE GENE FlaA

This document describes the method for the amplification of the gene coding for the
flagelline A. This method is an additional test to the molecular serotyping of Listeria
monocytogenes (MI CEB 13, based on the Doumith et al, 2004 method). This method can be
performed in parallel of the molecular serotyping (MI CEB 13) but is required for the strains
that could be “discordant” (groups IIa and IIc).
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[EURL Lm] Circular E-mail N°2014/15 - Methods for Lm molecular serotyping

Dear Colleagues,
As announced during the last workshop, we dispatch to you the two molecular serotyping EURL Lm in-house methods for molecular serotyping of Listeria monocytogenes (Lm), dealing with:
- the determination of the serogroups (revision);
- the amplification of the flaA gene (new).
A note introducing these 2 methods is also included.
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[EURL Lm] Circular E-mail N°2014/14 - EFSA report on Lm baseline survey-part B

Dear Colleagues,

 

I am pleased to inform you that EFSA has just published the report of Part B “Analysis of factors related to prevalence and exploring compliance” of  the analysis of the baseline survey on the prevalence of Listeria monocytogenes in certain ready-to-eat foods in the EU, 2010-2011: see web link below.

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[EURL Lm] Circular E-mail N°2014/13 - Publications on EURL Lm database

Dear colleagues,

 

I am pleased to announce you the recent publication of 2 papers on the set-up of the EURL Lm database, one published in Journal of Microbiological Methods, and one in ANSES publication Euroreference: see enclosed.

 

Wishing you a nice continuation of summer,

With best regards,

Bertrand

 

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[EURL CPS] Circular E-mail N°2014/13 - Information on annual meetings of ISO/TC 34/SC 9 & CEN/TC 275/WG 6

Dear Colleagues,

 

Please find enclosed the report of the EURL representatives to the annual plenary meetings of ISO/TC 34/SC 9 and CEN/TC 275/WG 6, dealing with international and European standardization in food microbiology, which were held in Washington DC last June. Resolutions/recommendations taken are also appended.

 

I have represented EURL CPS at these meetings.

 

To note in particular:

1.       ISO meeting:

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[EURL CPS] Circular E-mail N°2014/12 -Report of the 2014 workshop

Dear Colleagues,

 

Please find enclosed the report of the 8th annual workshop of EURL/NRLs for CPS, which took place here from 4 to 6 June 2014.

 

Wishing you a good continuation of this summer,

With best regards,

Bertrand

 

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Dr Bertrand LOMBARD
Head of team "coordination of reference activities", manager of European Union-Reference Laboratories Listeria monocytogenes, Staphylococci and Milk

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