Bovine Brucellosis Control of indirect ELISA kits (Individual & pooled serum samples)
The present document describes a standard technique aiming at controlling the fulfilment of OIE and EU requirements regarding the standardisation of indirect enzyme-linked immunosorbent assays (iELISA) kits for the detection of antibodies specific to smooth Brucella species (especially B. abortus, B. melitensis and B. suis) in bovine individual sera or pools of sera.
Animal Brucellosis Control of Rose Bengal, Complement Fixation and Milk Ring-test antigens
The present document describes a standard technique aiming at controlling the fulfilment of OIE and EU requirements regarding the standardisation of diagnostic antigens for the detection of antibodies specific of smooth Brucella species (especially B. abortus, B. melitensis and B. suis):
- in animal individual sera by the Rose Bengal Test (RBT) and the Complement Fixation Test (CFT);
- in bovine pooled milk samples by the Milk Ring-Test (MRT).
Ovine contagious Epididymitis (Brucella ovis) Complement Fixation Test
The present document describes a standard technique aiming at detecting antibodies specific of Brucella ovis by the complement fixation test in ovine sera.